THB-7 [HB-7, HB7]細胞
數(shù)量： 大量
細胞形態(tài)： **樣
**類型： IgG1
生長狀態(tài)： 懸浮生長
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： HB-136?
運輸方式： 凍存運輸
THB-7 [HB-7, HB7]細胞Designations: THB-7 [HB-7, HB7]
Depositors: TF Tedder
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: THB-7 [HB-7, HB7]細胞immunoglobulin; monoclonal antibody; against a human OKT-10 like molecule; against CD38
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Comments: Animals were immunized with the BJAB human B cell line.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with a 45000 dalton OKT-10 like molecule on immature human lymphocytes, plasma cells and activated T cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium, 85%; fetal bovine serum, 15%
Temperature: 37.0°C
THB-7 [HB-7, HB7]細胞Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
References: 22717: Tedder TF, et al. THB-7 [HB-7, HB7]細胞Discontinuous expression of a membrane antigen (HB-7) during B lymphocyte differentiation. Tissue Antigens 24: 140-149, 1984. PubMed: 6440308
