131-94H4細(xì)胞
器官來(lái)源： 脾
細(xì)胞形態(tài)： **樣
**類型： IgG1 kappa
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
運(yùn)輸方式： 凍存運(yùn)輸
年限： *****
數(shù)量： 大量
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
品系： 129GIX+
131-94H4細(xì)胞ATCC Number： CRL-2739?
Designations: 131-94H4
Depositors: National Cancer Institute
Isotype: IgG1 kappa
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Strain: 129GIX+
Organ: spleen
Cell Type: hybridoma: 131-94H4細(xì)胞B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against a synthetic v-myb oncogene peptide
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: *****
Gender: female
Comments: Animals were immunized with a synthetic polypeptide with the sequence RRKVEQEGYPQESSKAG(C) corresponding to peptides 119 to 135 of the MYB oncogene formerly v-myb (residues 2 to 18). Spleen cells were fused with Sp2/0 myeloma cells. Using immunoblot techniques, the antibody recognizes a 25 kd protein in BM2 cells and a protein of approximately 100 kd in Molt 4 cells. Protein is also detected in selected embryo tissues.The IgG1 and IgG2b reactivities of the antibody with the v-myb peptide (119 to 135) were confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly reactive.
Propagation: 131-94H4細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium:131-94H4細(xì)胞 Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
