LL 47 (MaDo)細(xì)胞

ATCC Number： CCL-135?

細(xì)胞類型： 成纖維細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來源： 人

年限： 16 years

器官來源： 肺

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

細(xì)胞形態(tài)： 成纖維樣

數(shù)量： 大量

LL 47 (MaDo)細(xì)胞Designations: LL 47 (MaDo)

Depositors: RG Crystal

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: lung

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. LL 47 (MaDo)細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: November, 1975

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 7,12

D13S317: 9,11

D16S539: 11

D5S818: 12,13

D7S820: 8,10

THO1: 7,9.3

TPOX: 9,10

vWA: 14,16

Cytogenetic Analysis: normal human male; diploid

LL 47 (MaDo)細(xì)胞At passage 9, the stemline number was diploid with a stable karyotype. One chromatid break was noted in 50 metaphase cells. The karyotype contained a brightly fluorescent Y chromosome as revealed by the quinacrine mustard fluorescence technique. Giemsa banding indicated no cross-contamination.

Isoenzymes: G6PD, B

Age: 16 years

Gender: male

Ethnicity: Black

Propagation: ATCC complete growth medium: Ham's F12K medium, 85%; fetal bovine serum, 15%

Subculturing: Protocol: Remove medium. Add fresh 0.25% trypsin, 0.03% EDTA for 1 to 2 minutes. Remove the trypsin and incubate the culture 37C for 10 to 20 minutes with a small volume of trypsin, EDTA. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%

Storage temperature: LL 47 (MaDo)細(xì)胞liquid nitrogen vapor phase