Lec8細(xì)胞
ATCC Number： CRL-1737?
細(xì)胞形態(tài)： 上皮樣
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 倉(cāng)鼠
器官來(lái)源： 卵巢
運(yùn)輸方式： 凍存運(yùn)輸
Lec8細(xì)胞數(shù)量： 大量
生長(zhǎng)狀態(tài)： 單層生長(zhǎng)
Designations: Lec8 [originally named Pro-5WgaRVIII3D]
Depositors: P Stanley
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: monolayer
Organism: Cricetulus griseus
Morphology: epithelial
Source: Lec8細(xì)胞Organ: ovary
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Gender: female
Comments: This line is a mutant clone derived from the parental CHO clone Pro-5 (a proline auxotroph, see ATCC CRL-1781) by selection for resistance to wheat germ agglutinin.
The cells exhibit a drastic reduction in the transport of UDP-galactose into the Golgi compartment, and may be useful for studying the role of galactose residues in the function and compartmentalization of glycosylated macromolecules.
The cells are proline auxotrophs and must be grown in a medium that contains proline (40 mg/L).
The population contains Pro+ revertants at high frequency (approximately 1 in 250 cells), and should be cloned if it is desired to use the Pro- marker in complementation studies.
These cells may also be grown as a suspension culture if agitated. Keep the cell concentration between 2 x 104 and 1 x 106 cells/ml.
Propagation: Lec8細(xì)胞ATCC complete growth medium: Alpha minimum essential medium, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, let sit for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes or until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
References: 1202: Stanley P. Selection of specific wheat germ agglutinin-resistant (WgaR) phenotypes from Chinese hamster ovary cell populations containing numerous lecR genotypes. Mol. Cell. Biol. 1: 687-696, 1981. PubMed: 9279382
22486: Deutscher SL, Hirschberg CB. Lec8細(xì)胞Mechanism of galactosylation in the Golgi apparatus. A Chinese hamster ovary cell mutant deficient in translocation of UDP-galactose across Golgi vesicle membranes. J. Biol. Chem. 261: 96-100, 1986. PubMed: 3510203
