Homo sapiens細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

年限： Neonatal

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： PCS-100-013

細(xì)胞形態(tài)： 其他

是否是腫瘤細(xì)胞： 0

物種來源： 人

Name: Primary Umbilical Vein Endothelial Cells; Normal, Human, Pooled

Biosafety Level: 1

Homo sapiens細(xì)胞Shipped: frozen

Organism: Homo sapiens (human)

Source: Umbilical vein endothelium

Growth Properties: Adherent

Age: Neonatal

Morphology: Cobblestone appearance

Volume per Vial: 1 ml

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Related Products: Vascular Cell Basal Medium (ATCC PCS-100-030)

Endothelial Cell Growth Kit/BBE (ATCC PCS-100-040)

Endothelial Cell Growth Kit/VEGF (ATCC PCS-100-041)

Homo sapiens細(xì)胞Dulbecco's Phosphate Buffered Saline (D-PBS) (ATCC 30-2200)

Trypsin-EDTA for Primary Cells (ATCC PCS-999-003)

Trypsin Neutralizing Solution (ATCC PCS-999-004)

Gentamicin-Amphotericin B Solution (ATCC PCS-000-025)

Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)

Phenol Red (ATCC PCS-999-001)

Recomended Culture Conditions: See Product Information Sheet

Comments: The cells are cryopreserved in the second passage to ensure the highest viability and plating efficiency. ATCC primary pooled HUVECs are derived from 10 individual donors, minimizing the lot-to-lot variability associated with cells derived from single donors. Large lot sizes assure consistency for large-scale studies.

A complete solution to propagate pooled HUVECs in low serum conditions with or without the addition of human recombinant VEGF. Use of the Endothelial Cell Growth Kit-VEGF will support a faster rate of proliferation because of the presence of several purified human recombinant (rh) growth factors (rh VEGF, rh EGF, rh FGF basic and rh IGF-1) combined with heparin and hydrocortisone. Use of the Endothelial Cell Growth Kit-BBE, which contains Bovine Brain Extract Homo sapiens細(xì)胞(BBE), is recommended if a more "authentic," less defined culture medium is desired.

Applications: Drug screening, angiogenesis, tubule formation, immune response, arteriolosclerosis, hypertension, vascular wound healing, inflammation, tissue remodeling, vascular differentiation, oxidative stress, apoptosis, stent development

Viability: ≥ 50%

Population Doubling Capacity: ≥ 15 in complete growth medium

Sterility Tests: Bacteria and Yeast: Negative

Mycoplasma: Negative

Testing: Hepatitis B: Negative

Hepatitis C: Negative

Mycoplasma: Negative

HIV-1 and HIV-2: Homo sapiens細(xì)胞Negative