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產(chǎn)品名稱(chēng): pET-29a(+)
產(chǎn)品型號(hào):
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無(wú)相關(guān)文檔
簡(jiǎn)單介紹
pET-29a(+)的各批次質(zhì)粒菌株發(fā)貨前均經(jīng)過(guò)嚴(yán)格的多重驗(yàn)證，如存在質(zhì)量問(wèn)題，請(qǐng)?jiān)谑盏疆a(chǎn)品的三個(gè)月內(nèi)通知我司。收到pET-29a(+)后請(qǐng)短暫離心，取2μl轉(zhuǎn)化至對(duì)應(yīng)感受態(tài)中，挑取單克隆重新提取質(zhì)粒后使用。
pET-29a(+)
的詳細(xì)介紹
pET-29a(+)載體基本信息
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pET29a, pet 29a
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大腸桿菌蛋白表達(dá)
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高
|
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多克隆位點(diǎn)，限制性?xún)?nèi)切酶
|
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5371 bp
|
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T7
|
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5'-TAATACGACTCACTATAGGG-3'
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N-S, N-Thrombin, C-His
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Kanamycin
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BL21(DE3) 、BLR(DE3)、BL21(DE3)pLysS
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N term thrombin cleavage site; a,b,c vary by MCS;
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69871-3
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瞬時(shí)表達(dá) Transient
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組成型 Constitutive
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非病毒
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pET-29a(+)載體質(zhì)粒圖譜和多克隆位點(diǎn)信息
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Feature Name
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Start
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End
|
|
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T7_terminator
|
129
|
1
|
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T7_Terminal_primer
|
69
|
87
|
|
lacI
|
218
|
1857
|
|
T7_transl_en_RBS
|
321
|
305
|
|
lacO
|
366
|
339
|
|
T7_promoter
|
384
|
366
|
|
tet (300 - 563)
|
420
|
683
|
|
pBRrevBam_primer
|
491
|
472
|
|
tet (576 - 851)
|
1916
|
2191
|
|
ROP
|
2666
|
2857
|
|
pGEX_3_primer
|
2873
|
2851
|
|
pBR322_origin
|
3891
|
3272
|
|
KanR2
|
3997
|
4812
|
|
ORF
|
Start
|
End
|
|
|
ORF frame 3
|
297
|
1025
|
|
ORF frame 1
|
898
|
1857
|
|
ORF frame 1
|
3997
|
4812
|
|
Enzyme Name
|
Cut
|
|
XhoI
|
158
|
|
NotI
|
166
|
|
EagI
|
166
|
|
HindIII
|
173
|
|
SalI
|
179
|
|
SacI
|
190
|
|
EcoRI
|
192
|
|
BamHI
|
198
|
|
EcoRV
|
206
|
|
NcoI
|
212
|
|
KpnI
|
238
|
|
BglII
|
241
|
|
BstBI
|
268
|
|
NdeI
|
295
|
|
XbaI
|
333
|
|
BclI
|
1139
|
|
ApaI
|
1336
|
|
HpaI
|
1631
|
|
FspI
|
2207
|
|
NruI
|
4085
|
|
SmaI
|
4302
|
|
XmaI
|
4300
|
pET-29a(+)載體簡(jiǎn)介
The pET-29a-c(+) vectors carry an N-terminal S?Tag?/thrombin configuration plus an optional C-terminal His?Tag® sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No.69337-3).
pET-29a(+)載體序列hz-2077R AMP deaminase 1 腺苷單磷酸脫氨酶1抗體
hz-1456R A2AR/Adenosine A2a receptor 腺苷A2A受體抗體
hz-0094R AACT-Alpha1/A1ACT α-1抗胰糜蛋白酶抗體
hz-1507R AAK1 AP2關(guān)聯(lián)激酶1抗體
hz-2123R Cyp2-j3 細(xì)胞色素P450Ⅱj3抗體
hz-2128R OST-beta 有機(jī)溶質(zhì)轉(zhuǎn)運(yùn)蛋白OSTβ抗體
hz-3914R AANAT 芳香胺N-乙酰化轉(zhuǎn)移酶抗體
hz-2133R AT2R2 血管緊張素Ⅱ受體2抗體
hz-2137R Influenza A virus (Duck) 鴨流感病毒抗體
hz-2150R TNF-alpha 腫瘤壞死因子-α抗體
hz-1603R AARS2 丙氨酰tRNA合成酶2抗體
hz-2185R TDRD9/HIG1 缺氧誘導(dǎo)蛋白HIG1抗體
hz-2257R SIRT1/sirtuin 1 沉默調(diào)節(jié)蛋白1抗體
hz-2321R Spindly/CCDC99 亞砷鹽相關(guān)蛋白抗體
hz-2354R TBX-5 轉(zhuǎn)錄因子Tbx5抗體
hz-0096R AAT/Tryptase α-1抗胰蛋白酶抗體
hz-1510R AATK AATK細(xì)胞凋亡關(guān)聯(lián)酪氨酸激酶抗體
hz-2451R NMP-22 核基質(zhì)蛋白22
hz-1229R AATF 拮抗凋亡轉(zhuǎn)錄因子抗體
hz-1627R ABCA1/ABC1 腺苷三磷酸結(jié)合盒轉(zhuǎn)運(yùn)體A1抗體
hz-1761R ABCD1/CCL22 嗜酸粒細(xì)胞趨化蛋白22抗體
hz-1604R ABCB5 ATP結(jié)合蛋白家族5抗體
hz-1224R ABCB6 ATP結(jié)合蛋白家族6抗體
hz-1960R ABCF1 ATP結(jié)合盒蛋白家族GCN20F家族1抗體
hz-1231R ABCG1 三磷酸腺苷結(jié)合盒亞家族G1抗體
hz-0662R ABCG2/CD338 三磷酸腺苷結(jié)合轉(zhuǎn)運(yùn)蛋白G超家族成員2抗體
