pET-16b載體基本信息
| 別名: | pET16b, pet 16b |
|---|---|
| 質粒類型: | 大腸桿菌蛋白表達 |
| 表達水平: | 高 |
| 克隆方法: | 多克隆位點,限制性內切酶 |
| 載體大小: | 5711bp |
| 5' 測序引物: | T7 |
| 5' 測序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 載體標簽: | N-His, N-Factor Xa蛋白酶切位點 |
| 載體抗性: | Ampicillin |
| 備注: |
Hosts: E.coli. Related vectors: pBR322.
|
| 產品目錄號: | 69662-3 |
| 穩定性: | 瞬時表達 Transient |
| 組成型: | 組成型 Constitutive |
| 病毒/非病毒: | 非病毒 |


| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 316 | 188 | |
| T7_Terminal_primer | 256 | 274 | |
| T7_transl_en_RBS | 419 | 403 | |
| lacO | 464 | 437 | |
| T7_promoter | 482 | 464 | |
| pBRrevBam_primer | 585 | 566 | |
| lacI | 860 | 1951 | |
| tet (576 - 1190) | 2010 | 2624 | |
| ROP | 3263 | 3454 | |
| pGEX_3_primer | 3470 | 3448 | |
| Ampicillin | 5503 | 4643 | |
| AmpR_promoter | 5573 | 5545 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 1 | 619 | 1119 | |
| ORF frame 2 | 992 | 1951 | |
| ORF frame 1 | 2429 | 1734 | |
| ORF frame 3 | 2007 | 2624 | |
| ORF frame 2 | 5503 | 4643 |
| Enzyme Name | Cut |
|---|---|
| ClaI | 24 |
| HindIII | 29 |
| BamHI | 319 |
| XhoI | 324 |
| NdeI | 331 |
| NcoI | 392 |
| XbaI | 431 |
| BglII | 497 |
| BclI | 1233 |
| ApaI | 1430 |
| HpaI | 1725 |
| EagI | 2287 |
| NruI | 2322 |
| MscI | 2794 |
| PstI | 4961 |
| AatII | 5638 |
| EcoRI | 5709 |
The pET-16b vector (Cat. No. 69662-3) carries an N-terminal His?Tag® sequence followed by a Factor Xa site and three cloning sites. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below.
